Alexa Fluor 647 Dye Profile
Thermo Fisher Scientific™
628-640
Laser Line
660/20
Common Filter
650
Excitation Max
665
Emission Max
239000
Extinction Coefficient
0.33
Quantum Yield800
Molecular Weight
About Alexa Fluor 647
Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.
Spectra
Popular Alexa Fluor 647 Antibodies
| Company/SKU | Applications | Marker / Reactivity | Host / Isotype / Clone | Size / Price | Details |
|---|---|---|---|---|---|
|
BD Biosciences 558094 |
FC,IF | CD31 (PECAM-1) Human |
Mouse IgG2a M89D3 |
100 μg $316 |
View |
|
BioLegend 320014 |
ICS | FOXP3 Human,Rat,Mouse |
Mouse IgG1 κ 150D |
100T $370 |
View |
|
BioLegend 515406 |
ICS | Granzyme B Human,Mouse |
Mouse IgG1 κ GB11 |
100T $364 |
View |
|
BioLegend 320013 |
ICS | FOXP3 Human,Rat,Mouse |
Mouse IgG1 κ 150D |
25T $188 |
View |
|
BioLegend 126408 |
ICS | FOXP3 Mouse |
Rat IgG2b κ MF-14 |
100 μg $370 |
View |
|
Cell Signaling Technology 6709S |
FC | TCF7 Human,Mouse |
Rabbit IgG C63D9 |
100 μl $465 |
View |
|
BioLegend 100724 |
FC,IHC-Fr,3D-IHC,Spatial Biology | CD8a Mouse |
Rat IgG2a κ 53-6.7 |
100 μg $216 |
View |
|
BioLegend 101218 |
FC,3D-IHC,Spatial Biology | CD11b Mouse,Human,Cynomolgus macaque,Rhesus macaque |
Rat IgG2b κ M1/70 |
100 μg $232 |
View |
|
BioLegend 117312 |
ICC,FC,IHC,3D-IHC | CD11c Mouse |
Armenian Hamster IgG N418 |
100 μg $229 |
View |
|
BioLegend 123122 |
FC,IHC-Fr,3D-IHC | F4/80 Mouse |
Rat IgG2a κ BM8 |
100 μg $323 |
View |
| Search Antibodies | |||||
Popular Alexa Fluor 647 Secondary Antibodies
| Company/SKU | Applications | Marker / Reactivity | Host / Isotype / Clone | Size / Price | Details |
|---|---|---|---|---|---|
|
BioLegend 406526 |
FC | IgM Mouse |
Rat IgG2a κ RMM-1 |
100 μg $229 |
View |
|
BioLegend 410714 |
FC | IgG Fc Human |
Rat IgG2a κ M1310G05 |
100T $347 |
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|
BioLegend 405707 |
FC,3D-IHC | IgD Mouse |
Rat IgG2a κ 11-26c.2a |
25 μg $112 |
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|
BioLegend 405708 |
FC,3D-IHC | IgD Mouse |
Rat IgG2a κ 11-26c.2a |
100 μg $265 |
View |
|
BioLegend 405416 |
FC,IHC-Fr | IgG Rat |
Goat IgG Poly4054 |
100 μg $188 |
View |
|
BioLegend 348228 |
FC | IgD Human |
Mouse IgG2a κ IA6-2 |
100T $358 |
View |
|
BioLegend 405322 |
FC,IHC-P | IgG Mouse |
Goat IgG Poly4053 |
100 μg $183 |
View |
|
BioLegend 348227 |
FC | IgD Human |
Mouse IgG2a κ IA6-2 |
25T $159 |
View |
|
BioLegend 406525 |
FC | IgM Mouse |
Rat IgG2a κ RMM-1 |
25 μg $89 |
View |
|
BioLegend 407116 |
FC,IHC-P | IgG2a Mouse |
Rat IgG RMG2a-62 |
100 μg $194 |
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| Search Antibodies | |||||
Alexa Fluor 647 Conjugation Kits
| Product Type | Company/SKU | Applications | Size / Price | Details |
|---|---|---|---|---|
| Conjugation Kit |
Abcam ab269823-1mg |
N/A | 1 mg |
View |
| Conjugation Kit |
Abcam ab269823-3x10ug |
N/A | 3x10µg |
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| Conjugation Kit |
Abcam ab269823-3x100ug |
N/A | 3x100µg |
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| Conjugation Kit |
Abcam ab269823-100ug |
N/A | 100µg |
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| Conjugation Kit |
Thermo Fisher Scientific A20186 |
Labeling | 5 x 100 μg $484 |
View |
