About Janelia Fluor 635b, NHS ester

Janelia Fluor 635b, NHS ester has an excitation peak at 654 nm and an emission peak at 671 nm. Janelia Fluor 635b, NHS ester is a spontaneously blinking fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for DNA labelling using amine-terminated oligonucleotides. Also used to label oligonucleotides for super-resolution assays of transposase-accessible chromatin (ATAC-SMLM). Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments. Derived from Janelia Fluor® 635, SE.