Alexa Fluor 555
Alexa Fluor™ 555 (Ex-Max 555 nm/Em-Max 565 nm)
Alexa Fluor™ 555 (AF555, Alexa 555) has an excitation peak at 555 nm and an emission peak at 565 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor® 555 (ATT Bioquest) and iFluor® 560 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 555 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 555 nm
- Emission maximum
- 565 nm
- Optimal laser line
- 532
- Optimal filter
- 575/36
- Relative brightness
- 2 of 5
- Extinction coefficient
- 150000
- Molecular weight
- 535
- Commercial antibodies
- 7.8k from 14 suppliers
Alexa Fluor 555 has an excitation maximum at 555 nm and an emission maximum at 565 nm, a Stokes shift of 10 nm. Its extinction coefficient is 150000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is Alexa Fluor 555?
- Alexa Fluor™ 555 (AF555, Alexa 555) has an excitation peak at 555 nm and an emission peak at 565 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor® 555 (ATT Bioquest) and iFluor® 560 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 555 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.
- What laser excites Alexa Fluor 555?
- Alexa Fluor 555 is optimally excited by the 532 laser line. Its excitation maximum is 555 nm.
- What filter should I use to detect Alexa Fluor 555?
- A 575/36 filter captures the peak of the Alexa Fluor 555 emission near 565 nm with minimal spillover.
- How bright is Alexa Fluor 555?
- Alexa Fluor 555 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to Alexa Fluor 555?
- FluoroFinder tracks 7758 commercially available Alexa Fluor 555 conjugates, searchable by target, clone, host and application.