CF555
CF®555 (Ex-Max 555 nm/Em-Max 565 nm)
CF®555 from Biotium is a fluorophore with an excitation peak at 555 nm and an emission peak at 565 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 555, ATTO 550 and DyLight™ 550. In Flow Cytometry, R-PE is a brighter alternative to CF®555. In microscopy, it has been validated for use in super-resolution imaging by STORM.
Excitation & emission spectra
Key facts
- Excitation maximum
- 555 nm
- Emission maximum
- 565 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
- Relative brightness
- 2 of 5
- Extinction coefficient
- 150000
- Molecular weight
- 901
- Commercial antibodies
- 121 from 2 suppliers
CF555 has an excitation maximum at 555 nm and an emission maximum at 565 nm, a Stokes shift of 10 nm. Its extinction coefficient is 150000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is CF555?
- CF®555 from Biotium is a fluorophore with an excitation peak at 555 nm and an emission peak at 565 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 555, ATTO 550 and DyLight™ 550. In Flow Cytometry, R-PE is a brighter alternative to CF®555. In microscopy, it has been validated for use in super-resolution imaging by STORM.
- What laser excites CF555?
- CF555 is optimally excited by the 532 laser line. Its excitation maximum is 555 nm.
- What filter should I use to detect CF555?
- A 585/42 filter captures the peak of the CF555 emission near 565 nm with minimal spillover.
- How bright is CF555?
- CF555 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to CF555?
- FluoroFinder tracks 121 commercially available CF555 conjugates, searchable by target, clone, host and application.